The Eppendorf µCuvette G1.0 microvolume measuring cell is a high-quality cuvette made of aluminum and quartz glass. It is the perfect tool for measuring high concentrations in small volumes. Higher sample concentrations require a shorter optical path length for measurements. With an optical path length of only 1 mm, the µCuvette G1.0 features a light path that is ten times shorter than the light path of standard cuvettes. This allows nucleic acid and protein concentrations to be measured with high reproducibility in a much higher concentration range without prior dilution. Due to the hydrophobic coating on the quartz glass only 1.5 µL nucleic acid or 3 µL protein sample are required for precise formation of the liquid column. Self-absorption of the µCuvette G1.0 is very low, meaning that the entire measuring range of the photometer can be used.
In general, the lower the concentration of a sample, the longer the optical path length should be. The same physical model (Beer´s Lambert Law) claims, if the concentration of a sample is very high, the light path length being used should be shortened to ensure enough light can pass through the sample to reach the detector. Microvolume measurements with light path lengths equal or shorter than 1mm are recommended for concentrations above 25 ng/µL dsDNA. If you are not sure which cuvette is the right solution for your sample, please visit our “About Detection” overview and check with the Cuvette Navigator. Please notice that the BioSpectrometers and BioPhotometers both accommodate standard cuvettes and the µCuvette G1.0. Thus, Eppendorf provides the flexibility of choosing the optimal light path length to detect a wide variety of sample concentrations!