PT Infiniti Bioanalitika Solusindo hosted an expert clinical webinar titled “Unlocking Molecular Insight: Digital PCR for Monitoring Chronic Myeloid Leukemia (CML) and Nasopharyngeal Cancer (NPC)”, featuring Dr. Ahmad Rusdan Handoyo Utomo, Ph.D., focusing on precision oncology diagnostics.
Core Scientific Takeaways
- Absolute Nucleic Acid Quantification: Unlike quantitative real-time PCR (qPCR) which requires external standard curves, digital PCR (dPCR) partitions reactions into tens of thousands of individual nanoliter droplets, applying Poisson statistics to compute absolute target copy numbers directly.
- High Analytical Sensitivity for MRD: Minimal Residual Disease (MRD) monitoring in Chronic Myeloid Leukemia requires detecting rare BCR-ABL1 fusion transcripts below MR4.5 (0.0032% IS). Digital PCR provides superior precision at low copy numbers where standard qPCR exhibits increased coefficient of variation.
- Liquid Biopsy for Nasopharyngeal Cancer: Circulating Epstein-Barr Virus (EBV) cell-free DNA serves as a validated non-invasive biomarker for NPC screening, recurrence monitoring, and therapeutic response evaluation.
For questions regarding digital PCR instrumentation, contact our technical team via Kontak Kami.
